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KMID : 0380619830150020177
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Korean Journal of Food Science and Technology
1983 Volume.15 No. 2 p.177 ~ p.182
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Study on the Pattern of Starch Assimilation by Sporobolomyces holsaticus
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신동화/Shin, Dong Hwa
민병용/구영조/박완수/Min, Byong Yong/Koo, Young Jo/Park, Wan Soo
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Abstract
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Direct conversion of starchy materials to single cell protein of Sporobolomyces hotsaticus FRI Y-5 was investigated. Effect of yeast extract concentration on its cell growth showed that it could utilize more of starch in the medium containing 2.5 g/ℓ of yeast extract. In case of jar fermentor culture, the specific growth rate and cell yield of Sp. holsaticus on soluble starch were calculated to be 0.14 hr^(-1) and 0.425, respectively and its maximum cell concentration was 13.4 g/ℓ. After 80 hr of incubation time, 45.96% of starch was consumed and 45.1% of relative blue value was decreased. Reducing sugars in the starch medium seemed to increase from 4.06 g/ℓ to 6.08 g/ℓ and then to decrease. During fermentor culture, pH of medium was almost not changed in the range of pH 7.0±0.5. The optimal temperature and pH of Sp. holsaticus amylase activity were 40 and pH 7.5, respectively. It was shown from the effect of Tapioc a starch concentration on the cell growth that the optimal concentration of Tapioca starch for Sp. holsaticus was lower than that of soluble starch. FRI Y-5 cells settled much slower than Sp. holsaticus IFO 1032 cells and the viscosity vs cell concentration relationship was related to be linear.
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